Abstract
Harmful cyanobacterial blooms are a growing environmental problem worldwide in natural waters, and the biodegradation is found to be the most efficient method for removing microcystins (MCs) produced by harmful cyanobacteria. Based on the isolation of a promising bacterial strain of Sphingopyxis sp. USTB-05 for biodegrading MCs, a gene USTB-05-A (HM245411) that is responsible for the first step in the biodegradation of MCs was cloned and expressed for the first time. The cell-free extracts (CE) of recombinant E. coli DH5α containing USTB-05-A had high activity for biodegrading MCs. Based on an analysis of the liquid chromatogram-mass spectrum (LC-MS), the enzyme encoded by gene USTB-05-A was found to be active in cleaving the target peptide bond between Adda and arginine, and converting cyclic MC-RR and MC-YR) to linear MC-RR and MC-YR as their first degradation products, respectively, which offered direct evidence for the first step on the pathway of MCs biodegradation by Sphingopyxis sp. USTB-05.
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