Abstract
ABSTRACT The present study aimed to assess the occurrence of aflatoxins in thefoodstuffs most consumed by the population in Algeria. To determine theaflatoxin B1 (AFB1) levels of coffee samples marketed in Constantine province(Algeria), 43 samples of green and roasted coffee were purchased from localmarkets and coffee shops. The occurrence and concentration range of AFB1 wereinvestigated by a highly sensitive microtitre plate Enzyme-Linked ImmunosorbentAssay (ELISA) method. The detection and quantification limits were 0.2ng g−1and 0.5ng g−1, respectively. The results showed that all the coffeesamples were contaminated with AFB1 in the range of 1.004 ng g−1to 1.167 ng g−1. The highest range was observed in Beans-roastedcoffee samples. The authors recommend that surveys of mycotoxins in coffee, aswell as other agricultural products, should be continued. Restrictive regulationsmust be applied to ensure the protection of consumers.
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