First Report on the Genus Gyrinicola Yamaguti, 1938 from India with a Description of a New Species.

Abstract

Gyrinicola dehradunensis sp. nov. from the intestine of tadpoles of the small paa frog, Nanorana minica, from Dehradun (Uttarakhand), India is described and illustrated. Gyrinicola dehradunensis is the first species of the genus recorded from India and the second species recorded from Asia. Light microscopy was used for the identification of nematodes using BX53 DIC/BF Olympus research microscope with an attached DP27 digital camera. Drawings for the description of the new species were made from photomicrographs. For molecular studies, DNA was isolated using Qiagen, DNeasy® Blood and Tissue Kit and amplified using r-DNA ITS-18S, and ITS-1.58S primers. The new species is differentiated from the known species by the absence of larvated eggs and the presence of a tooth-like projection in the buccal region. N. minica is a new host record for the genus. Four r-DNA ITS1 sequences of the new species have been submitted to the NCBI Genbank.