Abstract

Bacterial wilt is one of major threats to eucalyptus plantations which may cause significant losses. Until now, study about bacterial wilt on Eucalyptus pellita in Indonesia has been very limited, especially about the presence of the pathogen on or in the seeds. This study aims to provide evidence of the existence of the R. solanacearum bacterium on or in E. pellita seeds. Detection of seed-borne bacteria is determined by several approaches such as (i) direct detection using universal and selective media in the laboratory, (ii) the nursery test, and (iii) species-specific molecular detection. The results of our study indicate that R. solanacearum can be detected from eucalyptus seeds using universal and selective media in the laboratory, nursery test, and molecular-based detection using the Enrichment PCR method. The bacterial inoculum is also proven to exist both on the surface of and inside the eucalyptus seeds. This is the first report that R. solanacearum is a seed-borne pathogen in E. pellita seeds. Previous studies in different agricultural systems show that the effective method used to control the pathogen is through seed treatments using biological, physical, and chemical approaches.

Highlights

  • Forest plantation in Indonesia has been rapidly increasing since the last few decades and reached 11 million hectares last year [1]

  • This study aims to provide evidence of the existence of the R. solanacearum bacterium on or in E. pellita seeds

  • These findings indicate the potential of bacterial wilt disease to be transmitted through seeds

Read more

Summary

Introduction

Forest plantation in Indonesia has been rapidly increasing since the last few decades and reached 11 million hectares last year [1]. The use of healthy (pathogen-free) seeds is one approach that can be taken to reduce the risk of disease. Take for example the bacterial wilt pathogen of eucalyptus, Ralstonia solanacearum. Bacterial wilt is one of major threats in eucalyptus plantations which can cause significant losses [7]. Study about bacterial wilt on E. pellita in Indonesia is still very limited, especially regarding the presence of the pathogen on or in the seeds. This study aims to provide evidence of the existence of the R. solanacearum bacterium on or in E. pellita seeds. This is important as in different pathosystems the pathogen can be effectively controlled through seed treatments

Objectives
Methods
Results
Conclusion

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.