First report of Tuberose mild mosaic potyvirus from tuberose (Polianthes tuberosa L.) in India

Abstract

A potyvirus was detected molecularly by reverse-transcription polymerase chain reaction (RT-PCR) and serologically by Ouchterlony’s double diffusion test from a tuberose (Polianthes tuberosa L.) plant with mild mosaic disease. Potyvirus group-degenerate primers specific to the core region of coat protein gene were used in RT-PCR and the subsequent amplicon was sequenced. The sequence shared highest 98% and 99% identities at nucleotide and amino acid level, respectively and had a close phylogenetic relationship with the sequence of Tuberose mild mosaic potyvirus (TuMMV). This is the first report of TuMMV being present in India.