First Report of the New Root-Lesion Nematode Pratylenchus sp. on Soybean in North Dakota

Abstract

Root-lesion nematodes (RLN; Pratylenchus spp.) are important nematode pests on soybean (Glycine max (L.) Merr.). In May 2015 and 2016, sixtwo soil samples were collected from a soybean field in Walcott, Richland County, ND. Nematodes were extracted from soil using a sugar centrifugal flotation method., revealing these two All the samples contained RLN from 125 to 2, 000and 350 root-lesion nematodes per kg soil, respectively. In 2016, four soil samples were collected from the same field and all the samples had root-lesion nematodes ranging from 300 to 2,000. One soil sample with 350 RLN lesion nematodes per kg soil was planted withto soybean cultivar Barnes in four replicates each in a pot (6.4-cm × 25.4-cm) with 500 g soil(n = 4). After 15 weeks of growth at 22°C in a greenhouse room at 22°C, the root-lesion nematode RLN population was found to have increased greatly. The final population density in soil was 1,518 ± 541 lesion nematodesRLN per kg soil. Soybean roots were rinsed with water and brown lesion...