Abstract
Strawberry (Fragaria × ananassa Duch.) has recently become an economically important fruit crop in Pakistan and is cultivated on approximately 1500 ha. A survey across Punjab, Khyber Pakhtunkhwa (KPK) provinces and Islamabad (Islamabad Capital territory) was conducted during March and April of 2015-2016 growing season revealed black leaf spot symptoms on strawberry leaves. Leaf spots were irregular in shape, 3 to 6 mm in diameter, and blackish brown. Disease incidence was found 17 to 55 % in visited fields. Portions of symptomatic leaf tissue (3 × 5 mm) were excised from the margins of lesions; surface disinfected with 2% sodium hypochlorite for 2 min, rinsed three times in sterile distilled water (SDW) and blotted dry. Leaf tissue was then placed on Czapek Dox agar (CDA) and incubated at 25 ± 2°C with a 12-h photoperiod for 3 to 6 days. Morphology of the 78 isolates was examined; colonies were initially white, then turned light to dark brown and greyish black and produced abundant aerial hyphae. Conidiop...
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
