First Report of Soybean mosaic virus and Soybean yellow mottle mosaic virus in Vigna angularis

Abstract

In 2015 and 2016, 203 samples of adzuki bean (Vigna angularis) exhibiting viral disease-like symptoms, such as mosaic, mottle, yellowing, dwarfing and leaf rolling, were collected from 18 cities/counties in Korea. Total RNA was prepared from a pooled sample using easy-spinTM Total RNA Extraction Kit (iNtRON Biotechnology, Seongnam, Korea) and used for high-throughput paired-end RNA sequencing. Raw data analysis was conducted by Illumina HiSeq 2500 System at the Theragen Etex Bio Institute (Suwon, Korea) and SG-VIPdb at SeqGenesis (Daejeon, Korea), respectively, as previously described (Lim et al. 2015). As expected, 239 and 10 contigs related to Bean common mosaic virus and Cucumber mosaic virus, which have been previously reported in adzuki bean, were obtained from the adzuki bean transcriptome, respectively. However, in addition to this, 143 and 8 contigs were attributed to two soybean viruses, Soybean mosaic virus (SMV) and Soybean yellow mottle mosaic virus (SYMMV), respectively. To confirm the presen...