Abstract
Pistachio, Pistacia vera is one of the most important cash crops in Iran that is scattered in arid and semi-arid regions. During a survey of plant-parasitic nematodes of pistachio in Ardakan city in Yazd Province, a species of root-lesion nematode was isolated and identified by morphological, morphometrical, and molecular methods as Pratylenchus oleae Palomares-Rius et al., 2014. This species was isolated from several pistachio trees rhizosphere regarding to molecular analysis, D2–D3 expansion segments of 28S rRNA was amplified by PCR and sequenced. The sequence was deposited in GenBank (Accession No. MW338666). Along with the related phylogenetic analysis, placed this species in a monophyletic clade with other Pratylenchus oleae isolates, based on Bayesian inference (BI) phylogeny. A PCR reaction with the P. oleae specific primer set produced a 547 bp fragment. This is the first report of P. oleae infecting Pistachio tree in the world.
Highlights
Pistachio, Pistacia vera is one of the most important cash crops in Iran that is scattered in arid and semi-arid regions
Pratylenchus oleae from pistachio in Iran: Naeini and Taheri using the tray method (Whitehead and Hemming, 1965) and after 48h the nematodes at the bottom of tray were washed with tap water and kept for both morphological and molecular analysis
The Iranian isolate of P. oleae is characterized by lip region consisting of three annuli and four incisures along with oblique lines in middle of body, tail subcylindrical to mild conoid like to other P. oleae isolates
Summary
Pistacia vera is one of the most important cash crops in Iran that is scattered in arid and semi-arid regions. During a survey of plant-parasitic nematodes of pistachio in Ardakan city in Yazd Province, a species of root-lesion nematode was isolated and identified by morphological, morphometrical, and molecular methods as Pratylenchus oleae Palomares-Rius et al, 2014. This species was isolated from several pistachio trees rhizosphere regarding to molecular analysis, D2–D3 expansion segments of 28S rRNA was amplified by PCR and sequenced. Root-lesion nematodes species are considered as the third most important group of plant-parasitic nematodes affecting world wide crop They are widely distributed in pistachio orchards and reduce yields (Castillo and Vovlas, 2007). The aim of this work was to provide morphological, morphometrically and molecular characters of P. oleae from Iran
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