Abstract
Ginger (Zingiber officinale Roscoe) is an economically important crop that is planted on 60,000 to 70,000 ha every year in Shandong Province, China. During June to July 2019, a bacterial disease was observed on ginger in Shandong Province. The disease incidence was about 15% in each of three fields in Rushan (36°41'-37°08'N, 121°11'-121°51'E). Symptoms included leaves chlorosis, basal root and stem rot, rhizome rot, and wilting of foliage. The symptoms were similar to those associated with bacterial wilt of ginger, caused by Ralstonia solanacearum (Smith) Smith, which is a devastating and destructive disease of ginger in China (Ren and Fang 1981). Bacterial colonies isolated from infected ginger rhizomes surface-disinfested with 1% NaClO were purified on nutrient agar (NA) medium. Eighteen isolates were obtained from 20 diseased tissue samples. Three virulent isolates (SDGR1, SDGR2, and SDGR3) that differed morphologically from strains of R. solanacearum were selected for characterization based on morphological, biochemical, and molecular analyses. The colonies were light yellow, circular, smooth, and convex on NA. Individual cells were rod-shaped with peritrichous flagella (Dong and Cai 2001). The isolates were Gram negative, facultatively anaerobic, negative for urease, oxidase, and pectate degradation, as well as positive for pectate degradation, catalase, lactose and asparagine utilization (Dong and Cai 2001). These characteristics were consistent with Enterobacter spp., a member of the Enterobacteriaceae. For further identification, bacterial DNA of each of the three isolates was extracted from pure culture grown in liquid nutrient broth (NB) medium on a shaker at 28°C for 16 h. The sequence of the 16S rRNA gene was amplified using primers 27F/1492R (Lane 1991). The three isolates had identical 16S rRNA sequences and the consensus sequence (GenBank Accession No. MT233300) was analyzed by NCBI BLASTn, revealing 99.8% identity with the sequence of the 16S rRNA gene from E. cloacae strain R8-2 (GQ406570) (Wang et al. 2008). Construction of a phylogenetic tree of 16S rRNA sequences using a maximum likelihood analysis showed that the three ginger isolates clustered most closely with E. cloacae. The pathogenicity of three isolates was tested on ginger plantlets and immature rhizomes (Nishijima et al. 2004). Fifteen plantlets for each of the three isolates were each injected with 0.3 ml of a bacterial suspension (107 to 108 CFU/ml) at the stem base. The same number of plantlets were injected with sterile water as a control treatment. Inoculated plants were placed in a chamber at 90% relative humidity (RH) and 28°C with a photoperiod of 16 h. Rhizomes were surface-disinfected in 1% NaClO for 3 min, rinsed in distilled water, then cut into segments. Fifteen segments for each of the three isolates were injected with 0.2 ml of bacterial suspension (107 to 108 CFU/ml). Control rhizomes were treated similarly with sterile water. All rhizomes were placed in a chamber at 90% RH and incubated at 30°C in the dark. The pathogenicity tests were conducted three times independently. Brown discoloration, chlorosis, and necrosis of leaves developed 2 to 3 weeks after inoculation of plantlets for all three isolates. Water-soaked, brown and rotted rhizome tissue developed about 1 week after inoculation of rhizomes for all three isolates. No obvious symptoms were observed on the control plantlets and rhizome segments. The bacterial isolates that caused symptoms on the inoculated plants were re-isolated and identified as E. cloacae based on the same morphological, biochemical, and molecular analyses. The pathogen was not isolated from the control plantlets and rhizomes. E. cloacae has been reported as the cause of ginger rhizome rot in the United States and mulberry wilt in China (Nishijima et al. 2004; Wang et al. 2008). To our knowledge, this is the first report of ginger rhizome rot caused by E. cloacae in China. Confirming the existence of this pathogen in this area will be useful to adopt effective field management measures to control this disease on ginger.
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