First report of Plum bark necrosis stem pitting-associated virus infecting grapevine in China

Deya Wang,Yang Yang,Qingliang Li,Yufeng Wu,Yifan Sun

doi:10.1186/s12985-020-01438-3

Deya Wang, Yang Yang + Show 3 more

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https://doi.org/10.1186/s12985-020-01438-3

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Abstract

BackgroundVirus disease is one of the main diseases in grapevine, and there has been no report on Plum bark necrosis and stem pitting-associated virus infecting grapevine in China.ObjectiveThe leaf samples of grapevine cultivar 'Cabernet Gernischt' were collected from Shandong province, which the leaves suffered from viral-like symptoms with spotting and crinkle.MethodsSmall RNA-seq combined with reverse transcription PCR (RT-PCR) were performed to detect the potential viruses in these field samples. Phylogenetic tree was constructed using the neighbor joining method in MEGA 5.1ConclusionsThis is the first report of PBNSPaV infecting grapevine in China, contributing to a better understanding of the epidemiology and host range distribution of this pathogen.

Highlights

Grapevine virus diseases affect the yield and quality of grape (Vitis vinifera) [1]
To confirm the presence of the seven viruses in these grapevines, seven pairs of specific primers were utilized to detect the symptomatic samples by reverse transcription PCR (RT-PCR) and DNA sequencing, which showed that amplified products have the expected sequence size of Grapevine virus A (GVA) and Plum bark necrosis stem pitting-associated virus (PBNSPaV) in the samples
This is the first report of PBNSPaV infecting grapevine in China

Summary

Conclusions

This is the first report of PBNSPaV infecting grapevine in China, contributing to a better understanding of the epidemiology and host range distribution of this pathogen.

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