Abstract
During February 2017, 20% of the chilli plants in fields at the Indian Agricultural Research Institute, New Delhi, India showed witches’ broom symptoms of suspected phytoplasma etiology. Phytoplasma association was confirmed by utilizing a nested PCR assay with universal primer pairs P1/P7 and 3F/3R that target the phytoplasma 16S rRNA gene. The chilli witches’ broom phytoplasma (CWBP) sequence (Acc. No. KY612251) shared 100% nucleotide sequence identity with the 16S rDNA sequences of identified strains of ‘Candidatus Phytoplasma trifolii’ subgroup D (16SrVI-D) phytoplasmas associated with Lens culinaris’ witches’-broom (Acc. No. KY439869), brinjal little leaf (Acc. No. KX284698), Brassica oleracea var. capitata witches’ broom (Acc. No. KX671553) and Apium graveolens white leaf (Acc. No. KX671551) in pair wise sequence comparisons. The CWBP strain also clustered with strains of ‘Candidatus Phytoplasma trifolii’ subgroup D in phylogenetic comparison analysis. This is the first report of association of ‘Ca. P. trifolii’ subgroup D with witches’ broom disease of chilli in India.
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