Abstract

During 2014 and 2015, severe symptoms of dieback were observed in a commercial orchard of red raspberry (Rubus idaeus L.) cv. Heritage in Tangancicuaro, Michoacan, Mexico. Symptoms consisted of brown cankers and dieback on canes, accompanied by reddening and wilting of foliage, at an incidence of 5%. Internal symptoms included a brown discoloration of vascular tissues. A total of 30 stem pieces (approximately 20 cm in length) were collected from 15 diseased plants. Sixty colonies grown on potato dextrose agar (PDA) at 25oC developed a white, aerial mycelium that became olivaceous gray at the surface after 5 days. Pycnidia were produced after 2-3 weeks on autoclaved pine needles placed on water agar and were solitary or aggregated, dark brown to black, uniloculate, of 320 to 417 μm in diameter. Conidiophores were hyaline, cylindrical, smooth, thin walled, and septate. Conidia were hyaline, fusiform, base subtruncate to bluntly rounded, smooth, thin walled, aseptate, of (13-) 16 to 19 (-21) x (4-) 4.5 to 5....

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