Abstract
Symptoms associated with yellows disease of eggplants were observed in fields of Meerut district of India. Total DNA extracted from symptomatic and asymptomatic plants was subjected to nested-PCR assay using phytoplasma 16S rDNA primers and generic PCR with universal begomovirus coat-protein (CP) gene primers. Amplicons of expected size were obtained from symptomatic plants in both PCR assays. No amplifications were observed from asymptomatic plants. The eggplant phytoplasma was identified as a member of group 16SrVI, while the begomovirus showed 99% of CP gene identity with other whitefly transmitted begomoviruses.
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