Abstract
Mirabilis jalapa and Chrysanthemum sp. plants showing little leaf symptoms were observed during 2010 in New Delhi, India. Nested-PCR assays using phytoplasma specific 16S ribosomal DNA primers resulted in amplicons with sequence similarity to members of 16SrII group. Phy- logenetic analysis confirmed this finding by showing the relatedness of both the little leaf phytoplasmas from the current study with previously reported 16SrII members. This is first report of M. jalapa and Chystanthemum sp. as hosts of a 16SrII (Peanut witches' broom) group phyto- plasma from India.
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