Abstract
In October 2019, samples of galled roots with rhizosphere soil were collected from declining Elaeocarpus decipiens in Hernando County, Florida. Extracted root-knot nematodes were identified by both molecular and morphological methods as Meloidogyne enterolobii. This is a first report of this regulated root-knot nematode on Elaeocarpus decipiens in Florida.
Highlights
Elaeocarpaceae, Elaeocarpus decipiens, Guava root-knot nematode, Japanese blueberry tree, Meloidogyne enterolobii, Pacara earpod tree root-knot nematode, Regulatory
Nematodes were extracted from both soil and roots and species identification was performed using FDACS-DPI’s standard protocol for M. enterolobii, a COI-based qPCR assay (Kiewnick et al, 2015; BraunKiewnick et al, 2016) with slight modifications: Applied Biosystems QuantStudio 5 platform and SensiFast Lo-Rox chemistry, 40 PCR cycles instead of 45 cycles, annealing time of 30 s instead of 60 s, and an IDT produced custom oligonucleotide positive control is used instead of pure extracted M. enterolobii
The COI-based qPCR assay was repeated for positive samples, but with J2 extracted directly from roots
Summary
Elaeocarpaceae, Elaeocarpus decipiens, Guava root-knot nematode, Japanese blueberry tree, Meloidogyne enterolobii, Pacara earpod tree root-knot nematode, Regulatory. In October 2019, a sample of soil and roots was collected from under an E. decipiens in Hernando County, FL and submitted for certification for Meloidogyne enterolobii (Yang and Eisenback, 1983) at the Division of Plant Industry, Florida Department of Agriculture and Consumer Services, Gainesville, FL (FDACS-DPI). Nematodes were extracted from both soil and roots and species identification was performed using FDACS-DPI’s standard protocol for M. enterolobii, a COI-based qPCR assay (Kiewnick et al, 2015; BraunKiewnick et al, 2016) with slight modifications: Applied Biosystems QuantStudio 5 platform and SensiFast Lo-Rox chemistry, 40 PCR cycles instead of 45 cycles, annealing time of 30 s instead of 60 s, and an IDT produced custom oligonucleotide positive control is used instead of pure extracted M. enterolobii.
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