Abstract
Little cherry disease (LChD), is one of the most important viral diseases of cherry worldwide. It is associated to two different viral species, Little cherry virus 1 (LChV-1) and Little cherry virus 2 (LChV-2). Productivity impact of LChD is reflected in the decrease in yield and fruit quality (Ruiz-Garcia et al. 2016). For LChV-1 (genus Velarivirus, family Closteroviridae) there is no known insect vector to date, thus, its presence worldwide is mainly associated to the propagation of infected plants. In 2015-2016, during southern hemisphere spring-summer vegetative season, a survey was carried out for virus and viroid detection in the main cherry producing regions in Chile. RT-PCR analyses did not show the presence of LChD-associated viruses using previously reported specific primer pairs (Rott and Jelkmann, 2001). Nineteen samples whose symptoms did not match with the detected viruses, were selected for NGS analyses. Small RNA extracts were prepared according to Giampetruzzi et al. (2012). Libraries wer...
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