Abstract
Brassica parachinensis L. H. Bailey (Cruciferae), known as Chinese flowering cabbage, is a leafy vegetable and cultivated extensively in southern China. In November 2018, a leaf spot disease was observed on 28 to 34% of B. parachinensis in commercial fields located in Zijin county, Guangdong, China. Symptoms first appeared as small chlorotic spots on leaves, which enlarged and coalesced to form irregular gray-white necrotic lesions. Lesions became thinner with black pycnidia in the mesophyll tissues as the disease progressed. Some lesions eventually perforated. Eight symptomatic leaf samples from eight individual plants were collected. Conidia were collected from each sample using sterile distilled water. The resulting conidial suspension was serially diluted with sterile distilled water and evenly spread onto potato dextrose agar (PDA) amended with 0.05% lactic acid (v/v). After one day incubation at 25°C, germinated conidia were individually removed from PDA using a fine needle and a stereomicroscope, p...
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