First report of leaf curling and yellowing caused by ageratum yellow vein virus in Phaseolus vulgaris in Okinawa Prefecture, Japan

Abstract

In November 2016, leaf curling and yellowing were observed in common bean plants on Ishigaki Island, Okinawa Prefecture, Japan. Those symptoms suggested a virus-like disease. PCR using specific primers for ageratum yellow vein virus (AYVV) yielded PCR products of the expected size from diseased plants. The complete nucleotide sequence of the DNA-A shared 99.9% identity with that of the AYVV-Ishigaki isolate. Further, the virus was transmitted by Bemisia tabaci Middle East-Asia Minor 1 and Mediterranean. This is the first report of the natural occurrence of AYVV in common bean plants.