First Report of Grapevine Syrah virus-1 in Grapevine in Spain

Abstract

Grapevine Syrah virus-1 (GSyV-1) is a member of the genus Marafivirus of the family Tymoviridae. Although GSyV-1 has been described to infect grapevine (Vitis vinifera L.) in the United States, Chile, Italy, France, Slovakia, the Czech Republic, Brazil, Hungary, South Africa and Turkey, it has not been clearly associated to any disease (e.g. Czotter et al. 2015; Glasa et al. 2015; Oosthuizen et al. 2016; Caglayan et al. 2017). In 2016, surveys were carried out in three grapevine producing areas of the Catalonian region, D.O. Montsant, D.O. Priorat and D.O. Terra Alta. Seven of the 80 samples collected were randomly selected to study the sanitary status of the vineyards by Next Generation Sequencing (NGS), using RNAseq TrueSeq Illumina technology. In one of the Garnacha cultivar samples (C-22) from the D.O. Priorat, showing virus-like symptoms such as reddening and rolling of leaves, the NGS data analysis showed the presence of GSyV-1. The 19,799,291 reads obtained by NGS were subjected to a host genome su...