Abstract
BackgroundEdwardsiella tarda, is a serious bacterial pathogen affecting a broad range of aquaculture fish species. The bacterium has also been reported as a human pathogen, however recent studies have dissociated the fish pathogenic Edwardsiella from those isolated from humans by placing them in a new species, E. piscicida. Here we report the first case of Edwardsiellosis in cultured sharpsnout sea breams, Diplodus puntazzo in Greece.Case presentationThe disease has affected cultured sharpsnout sea breams of a commercial fish farm in a single location in East Greece. Two populations of sharpsnout sea breams stocked in two consecutive years in floating cages presented signs of disease which included nodules and abscesses in spleen and kidney, morbidity and cumulative mortality reaching 5.3 %. Using microbiological, biochemical and molecular tools we have identified Edwardsiella sp. as the main aetiological factor of the disease. Following phylogenetic analysis the bacterial isolates are grouped with the newly described Edwardsiella piscicida species.ConclusionsThis is the first report of Edwardsiellosis in this species but most importantly in sea cage-cultured fish in the Mediterranean which may pose a serious threat for aquaculture fish species in this region.
Highlights
Edwardsiella tarda, is a serious bacterial pathogen affecting a broad range of aquaculture fish species
This is the first report of Edwardsiellosis in this species but most importantly in sea cage-cultured fish in the Mediterranean which may pose a serious threat for aquaculture fish species in this region
Edwardsiella tarda is a serious bacterial pathogen affecting a wide range of fish species mostly in warm waters [1]
Summary
Edwardsiella tarda is a serious bacterial pathogen affecting a wide range of fish species mostly in warm waters [1]. Its production remains low [11] mostly due to the vulnerability of species to various infectious and non-infectious diseases [12,13,14] In this communication we report the isolation and partial characterization of E. tarda from diseased cultured sharpsnout sea breams. Pure cultures of two strains (EtS1 and EtS2) isolated from sharpsnout sea bream from each two incidences were sent to the laboratories of HCMR for further characterization. The isolates were Gram negative, non-motile, oxidase negative, rods, identified as E. tarda with 55 % probability using BIOLOG GENIII They could utilize several carbon sources such as sugars (maltose, glucose, N-Acetyl-Dglycosamine, N-Acetyl-neuraminic acid, mannose, fructose, glucose-6-PO4, fructose-6-PO4, serine), aminoacids (galacturonic acid, gluconic acid, glucuronic acid) and caroboxylic acid (lactic acid). Strains E. tarda (DSM 30052) and E. hoshinae (DSM 13771) were used as negative controls and Aeromonas veronii strain (Aero1- HCMR pathogen collection) previously shown to be strongly haemolytic on fish blood
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