First Report of DHA-1 Producing Enterobacter cloacae Complex Isolate in Bulgaria.

Dobromira I Dimitrova,Petya B Stankova,Ivan G Mitov,Temenuga J Stoeva,Lyudmila B Georgieva,Kalina I Mihova,Rumyana D Markovska,Radka P Kaneva

doi:10.3897/folmed.61.e39349

Dobromira I Dimitrova, Petya B Stankova + Show 6 more

Open Access

https://doi.org/10.3897/folmed.61.e39349

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Abstract

of the present study was to reveal the characteristics of an. Susceptibility testing, conjugation experiments, isoelectric focusing, PCR and sequencing were carrying out. Of 176. To the best of our knowledge this is the first report of DHA-1 producing isolate in Bulgaria. The emergence of DHA-1 producing.

Highlights

Species from Enterobacter cloacae complex can cause a wide range of nosocomial infections including respiratory infections, bloodstream infections (BSIs), urinary tract and surgical site infections.[1,2] In recent years, the level of resistance towards many antibiotic groups, especially beta-lactams, has increased due to acquisition of extended-spectrum beta-lactamases (TEM, SHV, CTX-M-) or carbapenemases (KPC, OXA-48, NDM).[3]
Conjugation experiments were carried out with rifampicin-resistant Escherichia coli K12:W3110 Rif R lac(-) strain, in solid cation-adjusted Mueller-Hinton agar as described previously.[7] β-lactamases were characterized by analytical isoelectric focusing (IEF) with the laboratory prepared polyacrylamide gel with Pharmalyte pH 3.5 -9.5 (Amersham-Pharmacia), as previously described.[7] β-lactamases bands were visualized by staining with a 500 mg/L solution of nitrocefin (BD Biosciences)
CMY and DHA are the most common groups of plasmid AmpC enzymes identified in Enterobacter spp.[1,10-18]

Summary

Introduction

Species from Enterobacter cloacae complex can cause a wide range of nosocomial infections including respiratory infections, bloodstream infections (BSIs), urinary tract and surgical site infections.[1,2] In recent years, the level of resistance towards many antibiotic groups, especially beta-lactams, has increased due to acquisition of extended-spectrum beta-lactamases (TEM-, SHV-, CTX-M-) or carbapenemases (KPC, OXA-48, NDM).[3]. Species from Enterobacter cloacae complex can cause a wide range of nosocomial infections including respiratory infections, bloodstream infections (BSIs), urinary tract and surgical site infections.[1,2]. The level of resistance towards many antibiotic groups, especially beta-lactams, has increased due to acquisition of extended-spectrum beta-lactamases (TEM-, SHV-, CTX-M-) or carbapenemases (KPC, OXA-48, NDM).[3]. Additional challenges are the chromosomal class C beta-lactamases (AmpC) characteristic for Enterobacter spp.[4]. The treatment of infections caused by these organisms with 3rd generation cephalosporines can cause an induction of the AmpC production, which, combined with ampD gene mutation, can result in stable high level production of these enzymes (the strains are known as “derepressed mutants”).[4]. In some cases the chromosomal AmpC gene has been mobilized on mobile elements and transmitted with them.

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Conclusion