First Report of Brown Stripe of Saltgrass Caused by Bipolaris heveae in Colorado.

Abstract

Inland saltgrass (Distichlis spicata var. stricta (L.) Greene) is indigenous to western North America and Australia and is a dioecious, rhizomatous, perennial, warm-season grass. It is commonly found in areas where salinity, alkalinity, and drought have eliminated many other types of vegetation (1). It has potential for revegetation of mine spoils or use along roadsides (2). During September 2004, multiple lenticular, brown lesions were observed on leaves of saltgrass accession no. 1023 at the Horticulture Field Research Center, Colorado State University, Fort Collins. Segments of symptomatic leaf tissue were surface sterilized in 0.5% sodium hypochlorite and placed on one-quarter-strength potato dextrose agar and incubated at 25°C in the dark. Dark green fungal colonies with aerial mycelium consistently grew on the medium. Slightly curved, ellipsoidal, pale-to-golden brown, smooth conidia 46 to 80 μm long and 13 to 17 μm wide (average 64.5 × 14.7 μm) with 6 to 9 septations formed after 7 days in cultures grown on V8 juice agar. The morphology and bipolar germination of conidia was consistent with the genus Bipolaris, however, conidia were often shorter than previously reported (3). The rDNA internal transcribed spacer (ITS) regions of one isolate were amplified using polymerase chain reaction (PCR) with universal fungal rDNA primers ITS1 and ITS4. PCR products were sequenced (555 bp) and exhibited 99% nucleotide identity to Bipolaris heveae isolates collected from zoysiagrass and bermudagrass in Japan (3) and rubber in Nigeria (4). To confirm pathogenicity, a suspension of 104 conidia per ml of water containing 0.1% Tween 20 was sprayed on saltgrass leaves to runoff. Plants were covered with transparent plastic bags and incubated at 25°C in the dark. After 72 h, the bags were removed and plants were placed in the greenhouse. Brown stripe symptoms were observed on all plants after 7 days, and B. heveae was consistently isolated from symptomatic tissue. To our knowledge, this is the first report of brown stripe on inland saltgrass caused by B. heveae.