Abstract
The seedlings of Cedrus deodara (Roxb.) G. Don in Solan district of Himachal Pradesh, India showed symptoms of root rot in forest nurseries. Initial observations revealed rotting and blackening of tap root, lesions at crown portion and drooping of 1 month old seedlings. Morphological and microscopic observations revealed the presence of binucleate Rhizoctonia AG-E. The sequence of internal transcribed spacer ITS1-ITS2 region of ribosomal DNA from Solan isolate was amplified by polymerase chain reaction assay using universal primers ITS1 and ITS2 which yielded 706 bp amplicon. This sequence was deposited in Genbank and its comparison with other sequences revealed 99% sequence identity with EU591790 which is binucleate Rhizoctonia AG-E = Ceratobasidium sp. CAG6.
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