Abstract
In the fall of 2016 and spring of 2017 Ralstonia solanacearum was isolated from blueberry plants (Vaccinium corymbosum) at seven farms in six counties in Florida. Symptoms were bronzing of leaves, marginal leaf necrosis, and bacterial streaming. The symptomatic plants resembled blueberry plants infected with Xylella fastidiosa. Symptomatic tissue samples were removed, surface sterilized, cut into distilled water, and streaked onto triphenyltetrazolium chloride medium (Kelman, 1954). Mucoid colonies with red pigmented, egg-shaped pattern characteristic of R. solanacearum formed within 48 hr at 28°C. In the fall of 2016, all bacterial strains were isolated from the Southern highbush blueberry cultivar ′Arcadia′, but as we widened our search via extension communications with growers, strains were isolated from other highbush cultivars. Ralstonia strains were characterized into phylotypes and sequevars by sequencing both the Internal Transcribed Spacer (ITS) region of the 16S rRNA gene (AMB013: CATCCACCGCTTGT...
Published Version
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