Abstract
In November- December, 2016–2017 Bur cucumber (Sicyos angulatus) plants samples showing severe symptom of yellowing, mosaic and leaf curling were collected from Horticulture research centre (HRC), Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut. Polymerase chain reaction using coat protein gene specific primer (ToLCVAV1F and ToLCVAV1R) gave an amplification of ~ 750 bp, similar to leaf curl virus reported on different crops and weeds. The gene was sequenced and submitted to GenBank with MF471344. BLAST analysis showed the association of Begomovirus with 99% nucleotide sequence identity with Tomato leaf curl Palampur virus (ToLCPalV) strain India: UP: Matera: Tomato [KF157552]. Phylogenetic analysis also supported the relationship of the obtained sequence with other begomoviruses as they clustered in a separate monophyletic cluster with ToLCPalV-Karnataka, India (KY564204, KY564205), India; ToLCPalV-Varanasi, India (FJ931537, GQ225738); ToLCPalV-Matera (KF157552), India and ToLCPalV-Allipur, India (HQ848383). Sequence Identity matrix with other begomovirus sequences was found to be in range between (78 and 99%). To our knowledge, this is the first confirmed evidence of a begomovirus infecting weed bur cucumber (Sicyos angulatus) in India.
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