Abstract
In Pakistan, roses are commercially valuable as ornamentals and for perfumes essential oils, rose syrup, and herbal tea. During 2015-2016, circular to oval black spots (2 to 3 mm in diameter) were observed on roses in the cities of Islamabad and Rawalpindi. A total of 35 symptomatic leaves were surface sterilized with 1% sodium hypochlorite for 2 min and then rinsed three times with sterile distilled water. Excised segments (3 mm2) of surface sterilized tissue were transferred to potato dextrose agar (PDA) and incubated at 25 ± 2°C. After 5 days, white colonies developed from 25 samples and later turned black or golden brown. The conidia were borne singly or in short chains and were obpyriform to obclavate, 10-40 x 6.2-17.60 µm with 0 to 3 longitudinal and 2 to 6 transverse septa. The morphological characters matched those of Alternaria alternata (Fr.) Keissl (Simmons 2007). For confirmation of pathogenicity, three asymptomatic mature rose leaves were surface sterilized with 1% sodium hypochlorite and the...
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