First report of a stunt nematode Tylenchorhynchus zeae on corn in Gansu Province, China.

Zhi Peng Xu,Hui Xia Li,Bao Cang Ren,Jin Hui Ma,Chun Hui Ni,Yong Gang Liu

doi:10.21307/jofnem-2020-009

Abstract

During a survey of plant parasitic nematodes in 2019, at Gansu Province, China, the stunt nematode Tylenchorhynchus zeae was found parasitizing corn seedlings. Females, males and juveniles of T. zeae were observed on soil and roots samples of corn after processing and extraction. This population of stunt nematodes was identified based on morphological and by sequencing the ITS1 region of rDNA and D2/D3 fragments of the 28 S rRNA. The ITS1 and the D2/D3 sequences of this population, shared 99.00 to 99.05% and 99.43 to 99.73% of similarity with sequences corresponding to T. zeae in GenBank, respectively. This is the first report of T. zeae infecting corn in Gansu Province, northwest China.

Highlights

First report of a stunt nematode Tylenchorhynchus zeae on corn in Gansu Province, China: Xu et al.2012), and the internal transcribed spacer1 (ITS1) region of rDNA and D2/D3 fragments of the 28 S rRNA were amplified with universal primers rDNA2 and rDNA1.58 s (Szalanski et al, 1997), D2A and D3B (Castillo et al, 2003), respectively
Males (n = 20): L = 483.21 ± 29.78 (445.62528.02) μ m, a = 27.45 ± 2.1 (24.12-30.59), b’ = 4.80 ± 0.36 (4.24-5.34), c = 16.54 ± 0.98 (15.25-18.51), c’ = 2.29 ± 0.11 (2.07-2.51), stylet length 15.94 ± 0.47 (15.32-16.74) μ m, tail length 29.42 ± 1.28 (27.37-31.53) μ m, spicule length 18.20 ± 1.46 (15.65-21.00) μ m, gubernaculum length 7.30 ± 0.68 (6.64-8.66) μ m, M = 0.47 ± 0.02 (0.44-0.51), O = 0.16 ± 0.02 (0.13-0.18), MB = 0.61 ± 0.03 (0.54-0.67). The morphology of this population was in agreement with the original population of Tylenchorhynchus zeae identified in India (Sethi and Swarup, 1968)
According with our morphological and molecular studies, this population was identified as T. zeae

Summary

Introduction

First report of a stunt nematode Tylenchorhynchus zeae on corn in Gansu Province, China: Xu et al.2012), and the internal transcribed spacer1 (ITS1) region of rDNA and D2/D3 fragments of the 28 S rRNA were amplified with universal primers rDNA2 and rDNA1.58 s (Szalanski et al, 1997), D2A and D3B (Castillo et al, 2003), respectively. First report, Northwest China, Stunt nematode, Tylenchorhynchus zeae. 5 females and 13 juveniles were obtained from 100 g soil, and 18 males and 2 females were extracted from 50 g of roots.

Results

Conclusion