First Report of a Novel Extended-Spectrum Beta-Lactamase KOXY-2 Producing Klebsiella oxytoca that Hydrolyses Cefotaxime and Ceftazidime

Abstract

Klebsiella oxytoca strains MU946294N and MB193997E were isolated from patients in Scotland. Strain MU946294N was resistant to pencillins, monbactams and cephalosporins. Isolate MB193997E displayed a β-lactam resistance phenotype consistent with chromosomal β-lactamase overproduction. No blaTEM, blaSHV or blaCTX-M genes could be amplified in either strain; however, amplification by PCR was found with primers for the blaOXY-2 gene. This β-lactamase gene in MU946294N differed by one mutation from the all other blaOXY genes previously reported, with an amino acid substitution Alanine237 Threonine enhancing the binding of cefotaxime. Strain MB193997E showed mutations at positions 255 and 283, neither of which affect function. Based on rpoB and gyrA characterization, both strains were assigned to the KoII phylogenic group but they were completely dissimilar from each other by PFGE. This study is the first to report the substitution of Alanine to Threonine at position 237 in a OXY-2 β-lactamase and this enhances resistance to cefotaxime.