First Report of a Mixed Infection of Youcai mosaic virus and Rehmannia mosaic virus in Rehmannia glutinosa in Korea

Abstract

Rehmannia glutinosa Libosch (family Scrophulariaceae) is a traditional medicinal herb in Asia, including China, Korea, and Japan, for general purposes. The vegetative propagation of the plant increase the incidence of virus infections in commercial fields, which causes major damages in R. glutinosa production in Korea. In September 2016, R. glutinosa showing mottling, yellowing, discoloration, and vein banding on leaves were found in an open field grown in Eumseong city, Korea. Electron microscopic observations from R. glutinosa revealed the presence of tobamovirus-like particles with an average particle size of ca. 310nm. Total RNA was extracted from the symptomatic leaves and analyzed by high-throughput Illumina RNA sequencing (Seo et al. 2015). A transcriptome library was generated using the TruSeq Stranded Total RNA with Ribo-Zero Plant kit (Illumina, San Diego, CA), according to the manufacturer’s instructions. The cDNA library was sequenced using Illumina HiSeq2000 system. The raw reads were quality...