Abstract
The carbapenemase OXA-244 is a derivate of OXA-48, and its detection is very difficult in laboratories. Here, we report the identification and genomic analysis of an Escherichia coli isolate (28Eco12) harboring the blaOXA-244 gene identified in Colombia, South America. The 28Eco12 isolate was identified during a retrospective study, and it was recovered from a patient treated in Colombia. The complete nucleotide sequence was established using the PacBio platform. A comparative genomics analysis with other blaOXA-244–harboring Escherichia coli strains was performed. The 28Eco12 isolate belonged to sequence type (ST) 38, and its genome was composed of two molecules, a chromosome of 5,343,367 bp and a plasmid of 92,027 bp, which belonged to the incompatibility group IncY and did not harbor resistance genes. The blaOXA-244 gene was chromosomally encoded and mobilized by an ISR1-related Tn6237 composite transposon. Notably, this transposon was inserted and located within a new genomic island. To our knowledge, this is the first report of a blaOXA-244–harboring Escherichia coli isolate in America. Our results suggest that the introduction of the OXA-244-producing E. coli isolate was through clonal expansion of the ST38 pandemic clone. Other isolates producing OXA-244 could be circulating silently in America.
Highlights
The World Health Organization WHO has recognized carbapenem-resistant Enterobacteriaceae as pathogens with critical priority for the development of new antibiotics [1]
92,027 bp (p28Eco12), which belonged to the of 5,343,367 bp and a plasmid of 92,027 bp (p28Eco12), which belonged to the incompatibility group incompatibility group not harbor resistance genes
This plasmid does not transport resistance genes, it appears to be conserved in almost all blaOXA-244 -containing E. coli strains included in our analysis, and its permanence is perhaps caused by the presence of the P1 phd-doc toxin-antitoxin system that participates in host post-segregational killing [12]
Summary
The World Health Organization WHO has recognized carbapenem-resistant Enterobacteriaceae as pathogens with critical priority for the development of new antibiotics [1]. OXA-244, a carbapenemase belonging to the Class D family, is a derivate of OXA-48 and encoded by the blaOXA-244 gene. There are multiple reports of OXA-48-producing isolates, reports of isolates harboring OXA-244 are less frequent, perhaps because their detection is difficult due to their reduced carbapenem activity. The blaOXA-244 gene was initially described in 2011, within a Klebsiella pneumoniae isolate, which was identified in Spain [2]. It has already been identified in Escherichia coli isolates recovered from. The molecular characterization of some of these E. coli isolates have shown that the majority of them belong to Antibiotics 2019, 8, 222; doi:10.3390/antibiotics8040222 www.mdpi.com/journal/antibiotics
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