First record of Pseudoterranova decipiens (Nematoda, Anisakidae) infecting the Red spot emperor Lethrinus lentjan in the Red Sea.

Saleh Al Quraishy,Mohamed Abdel Monem Dkhil,Rewaida Abdel-Gaber

doi:10.1590/s1984-29612019057

Saleh Al Quraishy, Mohamed Abdel Monem Dkhil + Show 1 more

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https://doi.org/10.1590/s1984-29612019057

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Abstract

The current parasitological study was carried out to investigate helminth parasites infecting the Red spot emperor Lethrinus lentjan inhabiting Hurghada City at the Gulf of Suez, Red Sea, Egypt. Third-stage larvae of nematode parasite was isolated from the intestine as well as body cavity of the examined fish. Light and scanning electron microscopy revealed that this parasite belonged to Anisakidae family within the genus Pseudoterranova. The present species is named Pseudoterranova decipiens based on the presence of triangular mouth aperture with prominent boring teeth and soft swellings of the cuticle, long muscular esophagus, ventrally excretory pore, and narrow transverse slit of anal opening followed by a short mucron. The morphological characteristics of this species were confirmed by molecular analysis of 18S rDNA gene region of the present parasite. It demonstrated a close identity ≥89% with taxa under family Anisakidae, 85% with Raphidascarididae, and 79-84% with Toxocaridae. A preliminary genetic comparison between gene sequence of the present parasite and other oxyurid species placeed it as a putative sister taxon to other Pseudoterranova decipiens described previously. This study demonstrated that the 18S rDNA gene region of Pseudoterranova decipiens yielded a unique sequence that confirmed its taxonomic position in Anisakidae.

Highlights

Anisakid nematodes have a global distribution among a wide variety of more than 200 marine fish species as intermediate host (MCCLELLAND et al, 1990) or as paratenic hosts (KØIE et al., 1995; KUHN et al, 2011)
Photomicrographs of Pseudoterranova decipiens third–stage larvae recovered during the period of this study from Lethrinus lentjan inhabiting Hurghada City at the Gulf of Suez, Red Sea, Egypt; showing high magnifications of: 1-3 Anterior end with boring tooth (BT), papillae (P), esophagus (E) and clear appearance of transverse annulations (TA) of the cuticle (C). 4 Transverse annulations (TA) of the cuticle
The anisakid nematode Pseudoterranova decipiens was recovered during the current study to infect the intestine as well as the body cavity of the Red spot emperor Lethrinus lentjan fish with 73.80% as a percentage of infection

Summary

Introduction

Anisakid nematodes have a global distribution among a wide variety of more than 200 marine fish species as intermediate host (MCCLELLAND et al, 1990) or as paratenic hosts (KØIE et al., 1995; KUHN et al, 2011). Cephalopod, marine mammals, and humans can become accidental hosts for anisakids by ingesting fish infected with third–stage larvae and their prevalence’s can be very high (WHARTON et al, 1999; ABOLLO et al, 2001; MCCLELLAND, 2002; SZOSTAKOWSKA et al, 2002). The life cycles of marine ascaridoid nematodes involve a number of stages.

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