Abstract
This paper presents the first geographical record of the needle nematode Longidorus artemisiae Rubtsova, Chizhov & Subbotin, 1999 outside Russia. This species was found in Poland near the city of Skierniewice in association with nettle (Urtica dioica L.). Morphometric and morphological data are provided, including the first description of juveniles of this species. Nematodes of the Polish population differ from the type-population in Russia in possessing a thicker body (lower ‘a’ index) in both sexes; males having a longer body and longer spicules; different sex ratio (1:2 in Polish population vs 1:1 in the type-population) and somewhat less expanded lips. Molecularly, the Polish population was characterised by sequencing D2-D3 28S rDNA and ITS1 markers. Additionally, new data on these two markers are provided for another species, Longidorus juglandicola Lišková, Robbins & Brown, 1997, obtained from topotype specimens from Slovakia. Surprisingly, despite the high morphological similarity of these two species, analysis of their phylogenetic position did not show close phylogenetic relation and several other species (less similar in general morphology) appeared more closely related to both L. artemisiae and L. juglandicola.
Highlights
The genus Longidorus Micoletzky, 1927 consists of obligatory plant ectoparasites, many of which are of economic importance as plant pests
Longidorus is rich in species (158 nominal species according to Peneva et al, 2013), which are discriminated mainly on the basis of morphology and morphometrics
This approach is complicated by high levels of intraspecific variability in morphometrics and minor interspecific differences leading to substantial overlap among species and increased potential for misidentification (Gutierrez-Gutierrez et al, 2013)
Summary
The genus Longidorus Micoletzky, 1927 consists of obligatory plant ectoparasites, many of which are of economic importance as plant pests. Longidorus is rich in species (158 nominal species according to Peneva et al, 2013), which are discriminated mainly on the basis of morphology and morphometrics. During a survey of the occurrence of the nematodes of the family Longidoridae in Poland, a previously non-recorded species Longidorus artemisiae Rubtsova, Chizhov & Subbotin, 1999 was found. This species was described from Russia on the basis of morphology and morphometrics of adult specimens. Longidorus juglandicola was described from Slovakia (Liskovaet al., 1997) from the rhizosphere of the walnut (Juglans regia L.) on the basis of morphology and morphometrics of adult and juvenile specimens. As no data on molecular markers of L. juglandicola were available, sequences from specimens of the type-population of L. juglandicola collected at the type-locality Sorozka (Slovakia) in 2015 are presented together with data on phylogenetic relationships of both species
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