Abstract

A potyvirus associated with mosaic disease of amaryllis was detected and identified by reverse-transcription and polymerase chain reaction using degenerate primers specific to the core region of coat protein gene of potyviruses followed by cloning and sequencing. The sequence shared highest (98–95%) amino acid identities with Amaryllis potyvirus that is not yet recognised by the International Committee on Taxonomy of Viruses, Amazon lily mosaic virus (tentative potyvirus) and Hippeastrum mosaic virus (recognised potyvirus). Screening bulbs of elite amaryllis cultivars for potyvirus infection would be useful to establish virus-free propagating material for the floriculture industry in India.

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