First microsatellite loci of the myxozoan parasite Tetracapsuloides bryosalmonae, the causative agent of proliferative kidney disease (PKD).

H Hartikainen,B Okamura,D Filippenko,A Vasemägi

doi:10.3354/dao02833

Abstract

Proliferative kidney disease (PKD) caused by the myxozoan parasite Tetracapsuloides bryosalmonae is a severe parasitic disease of salmonid fish. Estimates of genetic variation in parasite populations across Europe are currently lacking. We developed the first polymorphic microsatellite markers for T. bryosalmonae using Illumina MiSeq sequence data derived from genomic DNA. Twelve polymorphic loci were identified from 24 tested loci. Allelic variation was low at most loci, ranging from 2 to 6 (average 3.0). The markers developed here are expected to be useful in future genetic studies of T. bryosalmonae, aimed at further understanding the dispersal of the parasite, host-parasite relationships and the epidemiology of PKD.

Highlights

Tetracapsuloides bryosalmonae is a malacosporean (Phylum Cnidaria) parasite that causes proliferative kidney disease (PKD) of salmonids (Canning et al 1999)
We developed the first polymorphic microsatellite markers for T. bryosalmonae using Illumina MiSeq sequence data derived from genomic DNA
The markers developed here are expected to be useful in future genetic studies of T. bryosalmonae, aimed at further understanding the dispersal of the parasite, host−parasite relationships and the epidemiology of Proliferative kidney disease (PKD)

Summary

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Tetracapsuloides bryosalmonae is a malacosporean (Phylum Cnidaria) parasite that causes proliferative kidney disease (PKD) of salmonids (Canning et al 1999). PKD is considered as an emerging disease in the northern hemisphere, causing high mortality and significant losses of fish farm stock and impacting natural populations both in Europe and North America (Okamura et al 2011, Skovgaard & Buchmann 2012, Dash & Vasemägi 2014). Despite the economic and conservation implications of PKD, only a handful of sequences from T. bryosalmonae have been deposited in GenBank (mostly ribosomal DNA), and there are no polymorphic microsatellite loci available to describe the population genetic structure or identify multiple infections within hosts. By testing 24 tri- and tetranucleotide microsatellite markers from this library, we identified 12 polymorphic markers for F. sultana, none of which successfully amplified in T. bryosalmonae. BLAST searches of small subunit rRNA gene suggested that

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