Abstract
BackgroundPseudocowpox virus (PCPV) infects cattle worldwide with zoonotic potential but has not been isolated in Japan. Thus, the epidemiological status of PCPV infection in cattle is undetermined.ResultsIn May 2016, a cattle in a farm in Yamaguchi Prefecture showed white vesicles and hyperemia in the mucosa under the tongue surface, but not on the teats and coronary cushions. A parapoxvirus was isolated from the oral lesion swab and was genetically characterized based on the full-length sequence of B2L gene encoding viral envelope. Phylogenetic analysis showed that the isolated virus was classified into PCPV.ConclusionThis case indicates its potential spread in Japan. This is the first report of isolation of PCPV in Japan.
Highlights
Pseudocowpox virus (PCPV) infects cattle worldwide with zoonotic potential but has not been isolated in Japan
In Japan, serological surveys have revealed that seroprevalence of parapoxvirus is very high in cattle and sheep [3, 4] and multiple bovine papular stomatitis virus (BPSV) have been isolated [5], no PCPV has yet been isolated; the epidemiological status of PCPV infection in cattle is undetermined
Based on the nucleotide/amino acid identities and phylogenetic analysis of the full-length B2L gene, the YG2828 strain was classified as PCPV (Fig. 3)
Summary
Pseudocowpox virus (PCPV) infects cattle worldwide with zoonotic potential but has not been isolated in Japan. The epidemiological status of PCPV infection in cattle is undetermined. Conclusion: This case indicates its potential spread in Japan. This is the first report of isolation of PCPV in Japan. In Japan, serological surveys have revealed that seroprevalence of parapoxvirus is very high in cattle and sheep [3, 4] and multiple BPSVs have been isolated [5], no PCPV has yet been isolated; the epidemiological status of PCPV infection in cattle is undetermined. We determined the full-length sequence of the B2L gene encoding viral envelope of this isolate, and evaluated its phylogenetic relation to known members of this virus group
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
