Abstract
BackgroundThere have been an increasing number of infections in fish associated with different species of Chryseobacterium, being considered potentially emerging pathogens. Nevertheless the knowledge of the diversity of species associated with fish disease is partial due to the problems for a correct identification at the species level based exclusively on phenotypic laboratory methods.ResultsChryseobacterium shigense was isolated from the liver, kidney and gills of diseased rainbow trout in different disease episodes that occurred in a fish farm between May 2008 and June 2009. Identity of the isolates was confirmed by 16 S rRNA gene sequencing and phenotypic characterization. Isolates represented a single strain as determined by random amplified polymorphic DNA analysis.ConclusionsThis is the first description of the recovery of C. shigense from clinical specimens in trout, a very different habitat to fresh lactic acid beverage where it was initially isolated.
Highlights
There have been an increasing number of infections in fish associated with different species of Chryseobacterium, being considered potentially emerging pathogens
Bacterial strains and culture conditions The bacterial isolates were recovered from liver (635–08, 628-2-08; 692–08), kidney (664–09) and gills (706B-08, 972B-08, 1107B-09) of rainbow trout (Oncorhynchus mykiss) fry during five outbreaks (May, June and September of 2008 and June 2009) occurred in a fish farm located in the central region of Spain
Comparative analysis of the 16 S rRNA gene sequences revealed that the isolates shared 99.8-100 % sequence similarity between each other, demonstrating their high phylogenetic relatedness, 99.2-99.8 %, with the type strain of C. shigense (GUM-KajiT; Figure 1) and only 81.8-81.9 % with F. psychrophilum NCIMB 1947T (GenBank accession n D12670)
Summary
There have been an increasing number of infections in fish associated with different species of Chryseobacterium, being considered potentially emerging pathogens. Members the genus Chryseobacterium are widely distributed microorganisms that can be recovered from a wide variety of environments, such as fresh water, sewage and wastewater, soil or food sources, such as milk, poultry and meat and dairy products [1]. Some species of Chryseobacterium have been involved in human infections, acting as sporadic but severe opportunistic nosocomial pathogens [2,3]. Chryseobacteria are not relevant pathogens for domestic animals, but they are widely distributed in aquatic environments and fish farms [1,4]. Until recently members of the genus Chryseobacterium were not commonly associated with fish infections. Strains have been isolated from different fish species.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
