First investigation of Staphylococcus argenteus in a Brazilian collections of S. aureus isolated from bovine mastitis

Bruna F Rossi,Juliano L Gonçalves,Stéfani T A Dantas,Vera L M Rall,Rinaldo A Mota,Hélio Langoni,Marcos V Santos,Érika C R Bonsaglia,Ivana G Castilho,José C F Pantoja,Ary Fernandes Júnior

doi:10.1186/s12917-020-02472-7

Bruna F Rossi, Juliano L Gonçalves + Show 9 more

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https://doi.org/10.1186/s12917-020-02472-7

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Abstract

BackgroundStaphylococcus argenteus is a new specie positive coagulase staphylococci. We investigate the presence of S. argenteus in isolates previously classified as S. aureus, obtained from the milk of cows with mastitis in Brazil.ResultsAmong 856 S. aureus tested in chocolate agar, tryptone soya agar and salt egg yolk agar, white or colorless colonies were observed in 185 (21.6%) isolates. Regarding the ctrOPQMN operon, 111 (60%) presented the complete cluster. Despite some missing genes in this cluster, the remaining strains (74) were confirmed as S. aureus using the nrps gene.ConclusionsAs far as we know, this is the first review of S. aureus collection in Brazil and S. argenteus does not appear to be a significant problem in Brazilian herds.

Highlights

Staphylococcus argenteus is a new specie positive coagulase staphylococci
It can be caused by a variety of microorganisms and Staphylococcus aureus is one of the most important pathogens isolated from the milk of cows with subclinical mastitis [2]
Considering all genes that make up the staphyloxanthin operon, 74 out of 185 (40%) non-pigmented isolates did not present at least one of them, and crtO was the least frequent, according to Table 2

Summary

Introduction

Staphylococcus argenteus is a new specie positive coagulase staphylococci. We investigate the presence of S. argenteus in isolates previously classified as S. aureus, obtained from the milk of cows with mastitis in Brazil. Mastitis is a common disease in dairy herds, causing large declines in profitability and harmful effects on animal welfare [1]. It can be caused by a variety of microorganisms and Staphylococcus aureus is one of the most important pathogens isolated from the milk of cows with subclinical mastitis [2]. 10] and is predominantly human-associated, their recovery from several animals already occurred [3]. This new species had already been described before S. aureus like CC75/ST 75 [11], with an evolutionary sequence very distant from other S. aureus strains. S. argenteus presents more than 60 CC, such as CC2196 (35 STs), followed by CC1594 (12 STs), CC2198 (7 STs), CC75 (5 STs), and CC2793 (2 STs) [6, 12, 13]

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