Abstract
ABSTRACTClostridium oryzae was originally isolated from the soil of a Japanese rice field. C. oryzae represents a novel species within the genus Clostridium and is associated with anaerobic rice straw degradation. Here, we present the draft genome sequence of C. oryzae DSM 28571 (5.076 Mbp), containing 4,590 predicted protein-coding genes.
Highlights
Clostridium oryzae was originally isolated from the soil of a Japanese rice field
3 repeat regions were found in the genome of C. oryzae
Two of these regions were in close proximity to a clustered regularly interspaced short palindromic repeat (CRISPR)associated (Cas) gene cluster present in the genome
Summary
Clostridium oryzae was originally isolated from the soil of a Japanese rice field. C. oryzae represents a novel species within the genus Clostridium and is associated with anaerobic rice straw degradation. Chromosomal DNA was extracted applying the MasterPure complete DNA purification kit, according to the instructions of the manufacturer (Epicentre, Madison, WI, USA). Illumina paired-end seqeuncing libraries were prepared using the Nextera XT DNA library preparation kit, as recommended by the manufacturer (Illumina, San Diego, CA, USA). Sequencing of DNA was performed employing an Illumina MiSeq instrument and Illumina MiSeq reagent kit version 3, as described by the manufacturer. Trimming of reads using Trimmomatic version 0.36 (2) resulted in 2,856,526 paired-end reads.
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