First identification and characterization of porcine enterovirus G in the United States.

Fausto Baldanti,Srivishnupriya Anbalagan,Ben M Hause,Richard A Hesse

doi:10.1371/journal.pone.0097517

Abstract

Porcine enterovirus G (EV-G) is a member of the family Picornavirdae, genus Enterovirus. To date, eleven EV-G types (EV-G1 through EV-G11) have been identified in pigs from Asia and Europe however they have never been reported in North America. In this study, we isolated and characterized the complete genome of NP/2013/USA, an EV-G from a porcine diarrhea sample from the United States. The complete genome consists of 7,390 nucleotides excluding the 3′ poly(A) tail, and has an open reading frame that encodes a 2,169 amino acid polyprotein. NP/2013/USA was most similar at the nucleotide (84%) and amino acid (95%) level to the HM131607, an EV-G1 type isolated from China in 2012.

Highlights

Porcine enteroviruses (PEV) are members of the familyPicornaviridae, genus Enterovirus and were originally divided into serotypes (PEV-1 to -13) [1,2]
Further taxonomical studies led to the subdivision of PEV into three genera Teschovirus, Sapelovirus, and Enterovirus with porcine species porcine teschovirus (PTV), porcine sapelovirus (PSV) and porcine enterovirus B (PEV-B)
PEV-B has been renamed as enterovirus G (EVG), with the prototype EV-G viruses, PEV-9 and PEV-10, reclassified as EV-G1 and EV-G2, respectively [5,6]

Summary

Introduction

Porcine enteroviruses (PEV) are members of the familyPicornaviridae, genus Enterovirus and were originally divided into serotypes (PEV-1 to -13) [1,2]. We report the complete genome of an EV-G virus isolated from a clinical sample submitted to Newport Laboratories, Worthington, MN. EV-G was isolated from pigs with diarrhea, no association was identified between EV-G EV-G used in this study was isolated from a sample submitted to Newport Laboratories for routine diagnostic testing.

Results

Conclusion