First finding of Daidzein 7-O-phosphate and Genistein 7-O-phosphate that are hydrolyzed by sulfatase

Abstract

Attempted structural assignment of two water soluble isoflavone analogs of Daidzein and Genistein, was initially assumed to be the corresponding sulfates on the basis of the facts that these analogs were hydrolyzed by sulfatase; however, they were eventually determined through NMR technology including 31P–13C couplings to be the corresponding phosphates both at the 7-O-position; thus, Daidzein 7-O-phosphate [4H-1-benzopyran-4-one,7-hydroxy-3-(4-hydroxyphenyl)-, 7-phosphate] and Genistein 7-O-phosphate [4H-1-benzopyran-4-one, 5,7 –dihydroxy-3-(4-hydroxyphenyl)-, 7-phosphate].