Abstract
Pacific bluefin tuna (Thunnus orientalis) spawning adults, juveniles and larvae are all reported to occur in areas between Philippine Sea and Japan Sea. However, no DNA evidence has been generated to support this. In this study, Pacific bluefin tuna caught North of Polilio Island in the Philippine Pacific Seaboard, was identified through DNA barcoding using mitochondrial DNA D-loop region and cytochrome oxidase 1 gene. The results show clustering of Philippine-caught bluefin tuna D-loop and CO1 sequences with published reference sequences for T. orientalis and supported by pairwise distances <0.034. This study provides the first DNA evidence on the occurrence of T. orientalis in Philippine waters.
Highlights
Pacific bluefin tuna (Thunnus orientalis) spawning adults, juveniles and larvae are all reported to occur in areas between Philippine Sea and Japan Sea
While it is generally accepted that Pacific bluefin tuna, T. orientalis occur in northern Philippine waters, there has been no DNA analysis, which is considered a direct evidence of species identification, done far to support this
For D-loop sequence analysis, the phylogenetic tree (Fig. 3) inferred from Neighbour Joining method using Tamura 3-parameter model shows that the obtained D-loop sequence from the Philippine “bluefin tuna” belonged to the same clade as T. orientalis, supported by a significant bootstrap value of 100
Summary
Pacific bluefin tuna (Thunnus orientalis) spawning adults, juveniles and larvae are all reported to occur in areas between Philippine Sea and Japan Sea. Introduction Pacific bluefin tuna Thunnus orientalis, (Temminck & Schlegel, 1843) is considered as one of the most commercially important tuna species, highly priced reaching up to 100,000 US dollars per piece (Volpe 2005).
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