First determination and analysis of the complete mitochondrial genome of X-ray tetra Pristella maxillaris (Ulrey, 1894) (Actinopteri, Characidae)

Abstract

The complete mitochondrial genome of the X-ray tetra (Pristella maxillaris, Ulrey, 1894) was determined by using next-generation sequencing technology, and its mitochondrial genome characteristics were analyzed. The sequence total length was 16,753 bp, and the A + T content was 57.44%. The position and composition of the 37 genes were consistent with those of other Characidae species in this family. There are 13 protein-coding genes, 22 tRNA genes, two rRNA genes, and one control region. Except for ND2 and COX1, which use ATT and GTG as start codons, respectively, all other protein-coding genes use ATG as the start codon. COX1 uses AGG as the stop codon; ATP6 and COX3 use incomplete TA as the stop codon; COX2 and ND4 use incomplete T as the stop codon; ATP8 uses TAG as the stop codon, and the other seven protein-coding genes use TAA as the stop codon. Based on the concatenated nucleotide sequences of 13 protein-coding genes from 18 Characidae species, phylogenetic analysis revealed that P. maxillaris belongs to the family Characidae and is most closely related to Hyphessobrycon amandae. Determining the mitogenomes of P. maxillaris improves our understanding of the phylogeny and evolution of Characidae.