FIRST DETECTION OF PLASMID-MEDIATED 16S RRNA METHYLASES AND AMINOGLYCOSIDE MODIFYING ENZYMES IN AN EXTENDED SPECTRUM Î’-LACTAMASES PSEUDOMONAS AERUGINOSA CLINICAL ISOLATES FROM ALGERIA: A NEW BACTERIAL SUCCESS TOWARD PAN-DRUG RESISTANCE?

Abstract

Ponte Academic JournalMar 2020, Volume 76, Issue 3 FIRST DETECTION OF PLASMID-MEDIATED 16S RRNA METHYLASES AND AMINOGLYCOSIDE MODIFYING ENZYMES IN AN EXTENDED SPECTRUM Β-LACTAMASES PSEUDOMONAS AERUGINOSA CLINICAL ISOLATES FROM ALGERIA: A NEW BACTERIAL SUCCESS TOWARD PAN-DRUG RESISTANCE?Author(s): Addouda Abir ,Ammar Ayachi, Hafida Hassaine, Sana Dhaouadi, Leila Soufi, Messaoud Benmehidi, Ramzi Boubaker ElandalousiJ. Ponte - Mar 2020 - Volume 76 - Issue 3 doi: 10.21506/j.ponte.2020.3.19 Abstract:Abstract Introduction: The emergence of pan drug-resistant P. aeruginosa strains showing resistant to all antimicrobial agents is becoming a major public health concern. According to the previous hypotheses, these isolates acquired numerous drug-resistance determinants through the horizontal gene. To achieve this purpose, Our study conducted to characterize the genetic background of antibiotic resistance of Pseudomonas aeruginosa strains. Methodology: 51 no repetitive Pseudomonas aeruginosa strains were identified and investigated for genes encoding antimicrobial resistance determinants using polymerase chain reaction (PCR), besides, clonal relatedness of resistant isolates was analyzed by PCR ribotyping. Results: Various genes encoding antimicrobial resistance determinants were found in all isolates. In particular, the OXA-10Like gene in (44%) of strains, VIM-2 in (13,95%) and OprD in (4,65% ) ; molecular screening of 16S rRNA methyltransferase and aac (6 ') Ib was also found these genes in six strains, aac (3')Ia genes were detected in four strains. Ribotyping of resistant strains showed the presence of five different haplotypes. Conclusion: In this study, multiple antibiotic-resistance mechanisms contributed to the drug resistance of isolates were investigated. Thus, the genetic relationship between strains isolated from different units with the same genes resistance can explain the theory of strains dissemination and gene resistance in our hospital. Keywords: aminoglycoside-modifying enzymes; Clonal diversity; Pan drug-resistance; Pseudomonas aeruginosa; 16S rRNA methylase. Download full text:Check if you have access through your login credentials or your institution Username Password