First Detection of Carbapenemases-Producing Acinetobacter baumannii and Acinetobacter nosocomialis in Côte d’Ivoire

Abstract

Carbapenem resistant Acinetobacter baumannii are spreading worldwide and represent a major health threat for immunocompromised patients. In this study, we describe for the first time NDM-producing A. baumannii and Acinetobacter nosocomialis isolates from Côte d’Ivoire, Africa. Four carbapenem-resistant Acinetobacter spp. isolates were isolated from urinary tract infections from three university hospital centers. The antimicrobial susceptibility testing and carbapenemase detection (CarbAcinetoNP) test were performed. The carbapenemases genes were screened by PCR. Resistome, MLST and phylogeny were performed using whole genome sequencing. All isolates were resistant to β-lactams, aminoglycosides and fluroquinolones. The blaNDM-1 and the blaOXA-58 genes were identified in the three A. baumannii isolates whereas A. nosocomialis carried only the blaNDM-1 gene. An amino acid change S83L was detected in GyrA in all A. baumannii isolates explaining fluoroquinolone resistance. MLST analysis revealed that all A. baumannii isolates belonged to the same sequence type, ST-2. The blaNDM-1 and blaOXA-58 genes could not be transferred, suggesting a chromosomal location. This study highlights the emergence of NDM-1- and OXA-58-producing A. baumannii isolates belonging to the pandemic clone ST-2 in Côte d’Ivoire, Africa. Comparison of these three genomes revealed that they are highly related.