Abstract
Oryza coarctata plant, collected from Sundarban delta of West Bengal, India, has been used in the present study to generate draft genome sequences, employing the hybrid genome assembly with Illumina reads and third generation Oxford Nanopore sequencing technology. We report for the first time the draft genome with the coverage of 85.71 % and deposited the raw data in NCBI SRA, with BioProject ID PRJNA396417.
Highlights
Soil salinity is a major abiotic stress of rice cultivation globally (Molla et al, 2015), and rice cultivation areas under soil salinity stress are increasing gradually
To determine the genome size, 20 mg of young leaf tissue from Net house grown plants was chopped into small pieces and stained with RNase containing propidium iodide (50 μg/ml) (BD Science, India) as per the protocol of Dolezel et al (2007)
The O. coarctata genome (2n=4X=48; KKLL; Sanchez et al, 2013) is self-pollinated, (Sarkar et al, 1993) tetraploid plant with a genome size estimated by flow cytometry is found to be approximately 665Mb
Summary
Moose , University of Illinois at Urbana-Champaign, Champaign, USA. Any reports and responses or comments on the article can be found at the end of the article. In this revision, we have addressed all the issues raised by the referee. We revised the manuscript as a result of reanalysis of the data. We have improved clarity in the Methods regarding assembly of the genome. As per the suggestions of the referee as well as requirement, we incorporated 6 more references
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