Abstract
Iran bears a remarkable variety of reptiles. One of the lizard families occurring in Iran is the Family Agamidae which is widely are distributed throughout the old world. The large-scaled rock agamid, Laudakia nupta, is one of the well-known agamid. There are few reports of cloacal microbial on reptiles hence their function in cloacae remains unknown. Laudakia nupta usually live in rural and urban areas and close vicinity to man, they are likely to play an important role in the spread of disease that may be caused by these microorganisms and their transmission to man. Therefore, the aim of this study was to identify the bacterial flora colonizing the cloacal region of Laudakia nupta using molecular studies. The cloacal fluids were directly placed on nutrient agar (NA) plates and incubated at 25 ± 2 °C for 48 h. The resulting bacterial colonies were transferred to fresh nutrient agar (NA) plates for molecular studies. Twelve isolates were obtained from 17 specimens of Laudakia nupta. All bacteria isolates were identified as Bacillus subtillis (5), Bacillus cereus (4), Bacillus sp. (1), Pseudomonas putida (1), and Pseudomonas sp. (1) based on partial sequences of the 16 s rRNA gene. This is the first comprehensive report of bacteria spp. associated with cloaca of Laudakia nupta using molecular studies. In this research, we found that Laudakia nupta can be a carrier of bacteria which can transfer microorganisms to hosts.
Highlights
Reptiles are cold-blooded vertebrates among the oldest amniotes and are highly diverse in their morphology and ecological niches [1]
DNA extraction and PCR reaction, all bacteria isolates were identified as B. subtillis (5), B. cereus (4), Bacillus sp. (1), P. putida (1), and Pseudomonas sp. (1)
In conclusion, the results of this study indicate that rock lizard L. nupta harbor some bacteria in cloacal region
Summary
Reptiles are cold-blooded vertebrates among the oldest amniotes and are highly diverse in their morphology and ecological niches [1]. The large- scaled rock lizard is commonly referred to as Laudakia nupta. They are abundant on and among large rocks having deep crevices and around human habitation, commonly seen on walls, mud-brick dwellings, and the tombs and monuments of graveyards [3,4,5,6]. Reptiles have usually microbial contamination and asymptomatic carriers to transmission of microorganisms [7]. Even if appear healthy, often able to carry a wide variety of Sayyadi et al Laboratory Animal Research (2019) 35:3 pathogens for animals and humans [9]. Usually cause infections of gastrointestinal tract in human and animal [11,12,13]. Humans may come in contact with B. spp. B. cereus can result in intoxications that cause nausea, vomiting, and abdominal cramps or diarrhea [11,12,13]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
