Abstract
Cucurbit aphid-borne yellows virus (CABYV) was first described in France in 1992 and since then has been reported in various parts of the world, including the United States. Here, we present the first complete genome sequence of a CABYV isolate (BL4) that was collected from pumpkin during the 2017 growing season in Oklahoma.
Highlights
Cucurbit aphid-borne yellows virus (CABYV) was first described in France in 1992 and since has been reported in various parts of the world, including the United States
All seven genome fragments were amplified by reverse transcription-PCR (RT-PCR) with the respective primer pairs using total RNA as the template, as described previously [11]
Nucleotide sequences of each genome fragment were confirmed and assembled using DNASTAR Lasergene 13 (Madison, WI) software
Summary
Cucurbit aphid-borne yellows virus (CABYV) was first described in France in 1992 and since has been reported in various parts of the world, including the United States. We reported CABYV for the first time from commercial cucurbit fields in Blaine County in Oklahoma [10]. One of the dot-immunobinding assay (DIBA)-positive samples [10] against the CABYV antibody (designated as CABYV isolate BL4) was used in this work. Total RNA was extracted from the CABYV-infected leaf tissues of pumpkin [11].
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