Abstract
The 5′-terminal genomic sequence of Cherry virus A (CVA) has long been unknown. We determined the first complete genome sequence of an apricot isolate of CVA (7,434 nucleotides [nt]). The 5′-untranslated region was 107 nt in length, which was 53 nt longer than those of known CVA sequences.
Highlights
The 5=-terminal genomic sequence of Cherry virus A (CVA) has long been unknown
The cDNA library was constructed using the TruSeq RNA sample prep kit version 2 (Illumina, USA), and the library was paired-end sequenced by MiSeq (Illumina) with reagent kit version 2 (500 cycles)
The 5= end of the genome was amplified by rapid amplification of cDNA ends (RACE) using the Gene Racer kit (Invitrogen, USA)
Summary
The 5=-terminal genomic sequence of Cherry virus A (CVA) has long been unknown. We determined the first complete genome sequence of an apricot isolate of CVA (7,434 nucleotides [nt]). CVA possesses flexuous filamentous particles containing a monopartite, single-stranded, and positive-sense RNA genome with two open reading frames (ORFs). The draft genome sequence (7,383 nucleotides [nt]) of CVA was first reported in 1995
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