First Complete Genome Sequence of a Simian Foamy Virus Infecting the Neotropical Primate Brachyteles arachnoides.

Cláudia P Muniz,André F Santos,David H O’Connor,Dawn M Dudley,Liliane T F Cavalcante,Marcelo A Soares,Alcides Pissinatti,John J Dennehy

doi:10.1128/mra.00839-18

Abstract

The complete genome sequence of a simian foamy virus infecting the neotropical primate Brachyteles arachnoides (SFVbar) was obtained using next-generation sequencing and genome walking. The full-length SFVbar genome is composed of 11,994 bp and shows a genomic organization similar to that of other neotropical SFVs.

Highlights

The complete genome sequence of a simian foamy virus infecting the neotropical primate Brachyteles arachnoides (SFVbar) was obtained using nextgeneration sequencing and genome walking
The SFVbar sequence was aligned with the four New World primates (NWP) Simian foamy viruses (SFV) genome sequences available from GenBank (SFVsxa, accession number KP143760; SFVcja, accession number GU356395; SFVasp, accession number EU010385; and SFVspp, accession number GU356394) using MUSCLE in MEGA version 7.021
A nucleotide sequence comparison using the pairwise distance tool of MEGA showed that SFVbar is more similar to SFVasp in the pol gene (75.7% sequence identity) but less in env (68.8%) and in gag (55.8%)

Summary

Introduction

The complete genome sequence of a simian foamy virus infecting the neotropical primate Brachyteles arachnoides (SFVbar) was obtained using nextgeneration sequencing and genome walking. An 11,994-bp full-length genome of SFV infecting B. arachnoides was assembled.

Results

Conclusion